Transcriptome Analysis of Human Vascular Smooth Muscle Cells Cultured on a Polyglycolic Acid Mesh Scaffold

To construct tissue-engineered blood vessels (TEBVs) in vitro, it is necessary to transfer seed cells three-dimensional (3D) scaffolds for culture. However, what happens the behavior of after they are transferred scaffold unclear. Therefore, this study, a transcriptome analysis was used characterize differentially expressed genes (DEGs) vascular smooth muscle (VSMCs) before and 3D polyglycolic acid (PGA) understand changes functional gene expression early stage Transcriptome sequencing results showed that DEGs were mainly enriched cell proliferation cell-cell adhesion. The grown PGA (PGA-VSMCs) signal transduction. Furthermore, we found ERK1/2 significantly activated PGA-VSMCs inhibiting phosphorylation level ERK 1/2 increased elastin. In conclusion, material altered VSMCs affected elastin production. This study advances our understanding biomaterial-cell interactions provides valuable insights improving cultivation TEBVs.